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Fig. 8 | BMC Cancer

Fig. 8

From: Bile acids at neutral and acidic pH induce apoptosis and gene cleavages in nasopharyngeal epithelial cells: implications in chromosome rearrangement

Fig. 8

Caspase inhibitor reduces AF9 gene cleavages in bile acid-cotreated NP69 cells. a Representative gel pictures showing the IPCR detection of the AF9 gene cleavages in BA-treated NP69 cells: (i) without CI pretreatment (ii) with CI pretreatment. NP69 cells were left untreated or pretreated with 50 μM of Z-DEVD-FMK (Caspase-3 inhibitor II) for 1 h. The NP69 cells were subsequently left untreated (Lanes 1–6) or treated for 1 h with 0.5 mM of BA at pH 7.4 (Lanes 7–12) and pH 5.8 (Lanes 13–18). Genomic DNA was extracted and modified for nested IPCR as described in “Methods” section. The IPCR bands derived from the AF9 cleaved fragments were indicated by the side brackets. b Average number of AF9 gene cleavages detected in BA-treated NP69 cells. Data are presented as means and SDs of four independent experiments. Each experiment consisted of two to four sets of IPCR performed in three to six IPCR replicates per set for each cell sample. Values are expressed as fold change normalised to the value of the untreated control. The differences between the untreated control and treated groups were compared by using Student’s t-test. An asterisk indicates a significant increase in BA-treated cells as compared with the untreated control (* p < 0.05, ** p < 0.01). An open diamond represents a significant decrease in the sample with CI pretreatment as compared with its corresponding sample without CI pretreatment (p < 0.01)

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