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Fig. 4 | BMC Cancer

Fig. 4

From: Bile acids at neutral and acidic pH induce apoptosis and gene cleavages in nasopharyngeal epithelial cells: implications in chromosome rearrangement

Fig. 4

Bile acid triggers production of reactive oxygen species in NP69 cells. NP69 cells were treated with 0.5 and 1.0 mM of BA at pH 7.4 and pH 5.8 for 1 h. 20 mM of H2O2 was used as a positive control. After incubation with DCFH-DA, the cells were subjected to determination of ROS level and fluorescence microscopic analysis as described in “Methods” section. a Percentages of increased ROS level in NP69 cells after treatment with BA. Data are means and SD of five independent experiments performed in triplicate. Values are expressed in percentages with respect to values obtained from the untreated control. The differences between the untreated control and treated groups were compared by using Student’s t-test, * p < 0.01, ** p < 0.001. b Representative fluorescence microscopic images showing the BA-treated NP69 cells are shown. Magnification 100X, bar = 200 μm

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