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Fig. 2 | BMC Cancer

Fig. 2

From: Bile acids at neutral and acidic pH induce apoptosis and gene cleavages in nasopharyngeal epithelial cells: implications in chromosome rearrangement

Fig. 2

Bile acid induces mitochondrial membrane potential disruption in NP69 and TWO4 cells. NP69 cells were either left untreated or treated for 1 h with 0.5 mM of BA at pH 7.4 and pH 5.8. TWO4 cells were either left untreated or treated for 3 h with 0.5 mM of BA at pH 7.4 and pH 5.8. CPT was used as a positive control. After incubation, MMP disruption was assessed by flow cytometry in (a i) NP69 and (b i) TWO4 cells. Data presented are expressed as means and SD of two independent experiments performed in duplicate. The differences between untreated control and treated groups were compared by using Student’s t-test, * p < 0.05, ** p < 0.01. Representative contour plot diagrams showing the apoptotic populations of BA-treated NP69 (a ii) and TWO4 cells (b ii) determined by JC-1 staining are shown. The upper and lower quadrants represent the percentages of viable cells and apoptotic cells, respectively

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