Fig. 3

Establishment of putatively novel binding criteria in melanoma patients. a. Distribution of paired tumor (x-axis) and normal (y-axis) epitope binding affinities for all neoepitopes analyzed in the Hugo et al. [48] melanoma cohort. The vertical blue line divides epitopes into groups with (left) and without (right) strong tumor epitope binding (MHC affinity < 500 nM), while the horizontal blue line divides epitopes into groups with (bottom) and without (top) strong normal epitope binding (MHC affinity < 500 nM). The diagonal blue line divides epitopes into groups where the normal epitope binding affinity is at least 5× poorer than the tumor epitope (above) and where the normal epitope binding affinity is less than 5× poorer than the tumor epitope (below). Epitopes colored red are those that have strong tumor epitope binding affinities (< 500 nM), weak normal epitope binding affinities (> 500 nM), and normal epitope binding affinity at least 5× poorer than tumor epitope binding affinity (constituting a putatively novel binding change). Both axes are shown as log scale. b. Number of total (gray) and putatively novel binding (red) neoepitopes for each melanoma patient among the Hugo et al. cohort. Patients 15 and 21 each have a single putatively novel binding neoepitope. Y axis is shown as log scale