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Fig. 1 | BMC Cancer

Fig. 1

From: Population-level distribution and putative immunogenicity of cancer neoepitopes

Fig. 1

Illustration of proposed neoepitope prioritization metrics. a. Tumor vs. paired normal peptide binding affinity difference addresses the difference in MHC Class I binding affinity between the paired tumor and normal epitopes, and a novel binding change occurs when a tumor epitope binds readily to a patient’s HLA allele while its paired normal epitope does not. Examples are shown of a neoepitope which displayed a novel binding change (left) and a neoepitope which did not (right). Mutated residues are shown in blue underline. b. Tumor vs. paired normal peptide sequence similarity addresses the similarity in sequence between the paired tumor-normal epitopes at non-anchor residues based on a BLOSUM62 matrix, normalized by the tumor epitope’s similarity with itself. Examples are shown of a neoepitope with low similarity to its paired normal epitope (left) and a neoepitope with high similarity to its paired normal epitope (right). Anchor residue positions are shown faded, and mutated residues are shown in blue and underlined. c. Tumor vs. closest human peptide sequence similarity addresses how similar the neoepitope is to all human proteins based on a blastp search. Examples are shown of a neoepitope which matched to a peptide from a gene other than its gene of origin (left) and a neoepitope which matched to a peptide from its gene of origin (right). Anchor residue positions are shown faded, and mutated residues are shown in blue and underlined. d. Tumor vs. closest microbial peptide sequence similarity addresses how similar the neoepitope is to all bacterial and viral proteins based on a blastp search. Examples are shown of a neoepitope that matches closer to a microbial peptide than any human peptide (left) and a neoepitope which matches closer to a human peptide than any microbial peptide (right). Anchor residue positions are shown faded, and mutated residues are shown in blue and underlined

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