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Fig. 2 | BMC Cancer

Fig. 2

From: Oxidative stress enhances tumorigenicity and stem-like features via the activation of the Wnt/β-catenin/MYC/Sox2 axis in ALK-positive anaplastic large-cell lymphoma

Fig. 2

Converted RR cells share similar biological functions with native RR cells. a RU and RR cells from SupM2 cells were re-challenged with 0.3 mM H2O2 for 5 days. Then the cells were subjected to 0, 100, 200 ng/mL doxorubicin for 48 h. Cells without H2O2 treatment were included as control. b RU and RR cells derived from SupM2 cells were re-challenged with 0.3 mM H2O2 for 5 days. Then the cells were processed for methylcellulose colony formation assay in the presence/absence of 50 ng/mL doxorubicin, cells without H2O2 treatment were included as control. The graph demonstrated the number of colonies in the above experimental groups. The lower panel showed one representative result of triplicate experiments. Only colony with 40 cells (as pointed by the black arrow) or more was counted. c The serial diluted RU and RR cells derived from SupM2 were seeded in 96-well plate. After ~ 8 days, the number of spheres was counted in the highlighted wells circulated by the rectangle lines. The right panel showed the analyzed results which indicated that converted RR cells and native RR cells have formed more spheres in a lower number of cells seeded, in comparison with native RU cells

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