Fig. 4

G-Rh2 decreased the release and mRNA levels of VEGF and MMPs by lung cancer cells. a and b A549/H1299 and co-cultured with M2 derived from RAW264.7 cells were treated with G-Rh2(100 μM)for different time points as indicated. Supernatants were harvested and VEGF levels were measured by an ELISA kit. Experiments were repeated at least three times. *P < 0.05compared with the control. c A549 and co-cultured with M2 derived from THP-1 cells were treated with G-Rh2 (100 μM) for different time points as indicated. Supernatants were harvested and VEGF levels were measured by an ELISA kit. Experiments were repeated at least three times. * P < 0.05 compared with the control. d and e A549, Co-A549, H1299 and Co-H1299 cells were treated with different concentrations of G-Rh2 for 24 h. Cells were harvested for RNA extraction. The mRNA expression levels of VEGF-C, MMP9 and MMP2 were quantified by RT-PCR. * P < 0.05 compared with control. f A549 and co-cultured with M2 derived from THP-1 cells were treated with different concentrations of G-Rh2 for 24 h. Cells were harvested for RNA extraction. The mRNA expression levels of VEGF-C, MMP9 and MMP2 were quantified by RT-PCR. * P < 0.05 compared with control