Fig. 2
G-Rh2 inhibited the growth and migration of human lung cancer cells. a and b A549, H1299, andA549/H1299 cells co-cultured with RAW264.7 derived M2 macrophages (3 × 103/well) were treated with different concentrations (5, 10, 20, 40, 60, 80, 100 and 120 μM) of G-Rh2 for 72 h. Cell viability was estimated using CCK-8 assay. Experiments were repeated 3–5 times with the similar results. c A549 and A549 co-cultured with THP-1 derived M2 macrophages were treated with different concentrations (5, 10, 20, 40, 60, 80, 100 and 120 μM) of G-Rh2 for 72 h. Cell viability was estimated using CCK-8 assay. Experiments were repeated 3–5 times with the similar results. d A549 orco-cultured A549 cells were seeded in a 12-well plate to form a monolayer one day before the assay, and were scratched with a micropipette tip. After washed with PBS, the cells were treated with G-Rh2 (100 μM). The black lines indicate the wound edge; the blue lines indicate the recovery edge. Images for different times (0 h, 24 h, and 48 h) or the negative control (NC) are presented