Fig. 2

Effects of Reg IV and SOX9 on the invasion and migration abilities in MKN-45 cells. a Reg IV overexpression vectors, control empty vectors (PEGFP) and transfection reagents control (Mock) were added to the media. Cells were seeded on the 8 μm-filter chambers after 48 h, and harvested after 24 h. The invasive cells and migrated cells were photographed (top) and counted (bottom); (b) effects of Reg IV siRNA (siR-Reg IV) on invasion and migration of MKN-45 cells compared with Mock and control scramble siRNA (siR-NC); (d) in wound healing assay, cells were transfected with Reg IV overexpression vectors, control empty vectors (PEGFP), and transfection reagents control (Mock). After creating a confluent cell monolayer, cells were scraped in a straight and homogeneous line. The migration status were recorded every 12 h (top) and calculated (bottom); (e) effects of siR-Reg IV on migration of MKN-45 cells compared with Mock and control scramble siRNA; (f) effects of siR-SOX9 on migration of MKN-45 cells compared with Mock and control scramble siRNA. The results are shown as Mean ± SEM of 3 independent experiments. ^* P < 0.05, ^** P < 0.01, ^*** P < 0.001, N.S. = not significant