Fig. 8

The effects of MiaPaCa2 or AsPC1-cell supernatants on hepatic, fat, and skeletal-muscle metabolisms Normal mice in 3 groups (6 mice/group) were subjected to subcutaneous injection (0.5 ml, twice a day) of normal control medium (group N) or the media that were conditioned by MiaPaCa2 cells (group M) or by AsPC1 cells (group A). After 7 days, all mice were sacrificed. Their liver, fat, and skeletal muscle were obtained. Western blots were performed, using β-tubulin and β-actin as loading controls. a PCB and G6Pase expression in the liver. b ATGL expression in subcutaneous and epididymal fat. c Atrogin-1 and IGFBP-3 expression in skeletal muscle. Blots are representative results. The histograms show the results of all mice