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Fig. 2 | BMC Cancer

Fig. 2

From: Improved effects of honokiol on temozolomide-induced autophagy and apoptosis of drug-sensitive and -tolerant glioma cells

Fig. 2

Improved effects of honokiol on temozolomide (TMZ)-induced caspase-3 activation, DNA fragmentation, cell apoptosis, and cell necrosis. Human U87-MG glioma cells were exposed to honokiol (40 μM), TMZ (100 μM), and their combination for 72 h. The activity of caspase-3 was assayed using a fluorometric substrate method (a). DNA fragmentation was quantified with a cellular DNA fragmentation ELISA kit (b). Apoptotic cells were quantified using flow cytometry (c). Necrotic cells were determined with a photometric immunoassay (d). Each value represents the mean ± SEM for n = 6. * and #, values significantly (p < 0.05) differ from the respective control and TMZ-treated groups

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