Fig. 5

Amphotericin B inhibited the macrophage-mediated promotion of the proliferation and invasiveness of Raji cells. a Raji cells were co-cultured with macrophages pretreated with DMSO or amphotericin B, followed by the analysis of the cell proliferation of Raji cells by BrdU incorporation assay (***P < 0.001). b Cells were treated as a, and Raji cell invasion was assessed by Transwell assay (P < 0.05). c Cells were treated as a for 72 h, and the mRNA expression of IL-4, IL-6, IL-10, IL-1β and IL-13 in Raji cells was determined by RT-PCR. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Macrophage: macrophages alone without Amphotericin B treatment; Raji: Raji alone; Raji + Macrophages: macrophages without Amphotericin B treatment co-cultured with Raji cells; DMSO: macrophages with DMSO treatment first and then co-cultured with Raji cells; Amphotericin B: macrophages with Amphotericin B treatment first and then co-cultured with Raji cells