Fig. 4

IL-4 and IL-13 increased the expression of B7-H1 in macrophages. a Macrophages were treated with IL-4, IL6, IL10 and IL-13 for 24 h. The analysis of B7-H1 levels was determined by flow cytometry (upper panel) and western blotting (lower panel), respectively. b Macrophages were treated with 25 or 50 ng/ml of IL-4 or IL-13 for 24 h. p-STAT6 and B7-H1 were determined by immunoblotting. c Macrophages were treated with 25 or 50 ng/ml of IL-4 for 24 and 48 h, and p-STAT6 and B7-H1 were determined by immunoblotting. d Macrophages were treated with 25 or 50 ng/ml of IL-13 for 24 and 48 h, and p-STAT6 and B7-H1 were determined by immunoblotting. *P < 0.05, ** P < 0.01