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Fig. 3 | BMC Cancer

Fig. 3

From: Amphotericin B suppresses M2 phenotypes and B7-H1 expression in macrophages to prevent Raji cell proliferation

Fig. 3

Amphotericin B abolished Raji cells induced STAT6 phosphorylation in macrophages. a Raji cells were cultured with or without macrophages in the presence or absence of amphotericin B (1 μM) for 72 h, and STAT3 and STAT6 phosphorylation in macrophages were assessed by western blotting. Amphotericin B only inhibited the expression level of p-STAT6. b Cells were treated as a, and STAT3 phosphorylation in macrophages was detected by immunofluorescence (400×). Scale 50 μm. c Cells were treated as a, and STAT6 phosphorylation in macrophages was detected by immunofluorescence (400×). Scale 50 μm. Control: macrophages alone without Amphotericin B treatment; Raji + Macrophages: macrophages without Amphotericin B treatment co-cultured with Raji cells; Raji + DMSO + Macrophages: macrophages with DMSO treatment first and then co-cultured with Raji cells; Raji + Amphotericin B + Macrophages: macrophages with Amphotericin B treatment first and then co-cultured with Raji cells

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