Fig. 6

The effect of kinin receptor antagonism on MoCR cell invasion. MoCR cells were seeded into upper chambers with kinin/ kinin-receptor inhibitor combinations in lower chambers. The membrane on the lowers surface of the upper chamber was coated with fibronectin and allowed to set. a Non-treated cells, b Cells treated with 10.0 μM of SSR240612 (SSR) and 0.10 μM DABK, c Cells treated with 10.0 μM of FR173657 (FR) and 0.10 μM BK, [E] Number of cells that permeated the membrane. Cells were culture for 21 h in 0.01% BSA RPMI media. The control was 0.01% BSA RPMI media. SSR240612 and FR173657 treatment significantly reduced the number of invasive MoCR cells. Results are presented as replicate mean ± SD, 2 replicates per group (**p < 0.0001 compared to control). 40X magnification; Bar = 25 μm