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Fig. 3 | BMC Cancer

Fig. 3

From: Micro-RNA-186-5p inhibition attenuates proliferation, anchorage independent growth and invasion in metastatic prostate cancer cells

Fig. 3

Alteration of miR-186-5p levels affects PCa cell colony formation. Metastatic PC-3 and MDA-PCa-2b cells were transiently transfected with miR-186-5p inhibitor and mimic for 24 h and grown in 0.35% soft agar for 2–3 weeks at 37 °C. a Inhibition of miR-186-5p reduced PC-3 cell colony formation (p = 0.0033). b Ectopic expression of miR-186-5p increased in colony growth in LNCaP cells (p < 0.0001). c Representative images of anchorage-independent growth of PC-3 and LNCaP cells transfected with miR-186-5p inhibitor or miR-186-5p mimic, respectively. Data analyses were based on at least 3 independent experiments and presented as mean percentage ± S.D. (**p-value < 0.004, ****p-value < 0.0001)

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