Fig. 2

Inhibition of miR-186-5p reduced MDA-PCa-2b PCa cell proliferation. PCa cells were transiently transfected for 24–48 h with miR-186-5p inhibitor, mimic, and scramble negative controls. Cell proliferation was measured by the BrdU assay. a MiR-186-5p inhibition resulted in a significant decrease in MDA-PCa -2b (p = 0.013) relative to scramble control. b Ectopic miR-186-5p expression did not alter proliferation of PC-3 (p = 0.6744), MDA PCa2b (p = 0.4220) and LNCaP (p = 0.8582) relative to scramble control. Data analyses were based on 3 independent experiments and presented as mean absorbance values ± S.D. (*p-value < 0.02)