Table 1 Primers used for qRT-PCR, qCHIP and siRNAs oligonucleotides
From: Up-regulation of ceRNA TINCR by SP1 contributes to tumorigenesis in breast cancer
Primers used for qRT-PCR | |
|---|---|
TINCR-F | TGTGGCCCAAACTCAGGGATACAT |
TINCR-R | AGATGACAGTGGCTGGAGTTGTCA |
GAPDH-F | GCTCTCTGCTCCTCCTGTTC |
GAPDH-R | ACGACCAAATCCGTTGACTC |
siRNAs/shRNA oligonucleotides | |
scrambled | UUCUCCGAACGUGUCACGUdTdT |
si-SP1–1 | CAGCGUUUCUGCAGCUACCUUGACU |
si-SP1–2 | GACAGGUCAGUUGGCAGACUCUACA |
siTINCR-1 | UAUUCCUUCAGCCAGUACCCAGGUC |
siTINCR-2 | UUUCCAAGGUGGCACAGUGCUUUCC |
qCHIP analysis of the TINCR promoter for SP1 occupancy | |
BS3-F | TGACCTCGCTGATGGCTCT |
BS3-R | TCAGGCGTCCGCTCCCCACT |
BS1/2-F | TGAGGGGACCGTGGCA |
BS1/2-R | TGGTAGCGCTTCCAGCGCGACA |