Fig. 2
From: Up-regulation of ceRNA TINCR by SP1 contributes to tumorigenesis in breast cancer
TINCR-knockdown inhibited malignant progression in breast cancer cells. a MCF-7 and MDA-MB-468 cells were transfected with either scramble or TINCR siRNA by lipofectamine 2000. The knockdown efficiency was confirmed by real-time PCR 48 h post-transfection. ***p < 0.001; b, c Cell viability was determined in TINCR deficient MDA-MB-468 and MCF-7 cells by CCK-8 kit. d Soft agar assay was performed to evaluate the anchorage-independent growth in response to TINCR silencing, the statistical results from three individual fields were shown in pane (e). f, g The apoptotic cells were detected with Annexin-V/7-AAD double staining method, followed by flow cytometry analysis. **p < 0.01, ***p < 0.001