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Fig. 9 | BMC Cancer

Fig. 9

From: Knockdown delta-5-desaturase in breast cancer cells that overexpress COX-2 results in inhibition of growth, migration and invasion via a dihomo-γ-linolenic acid peroxidation dependent mechanism

Fig. 9

Mechanism of anti-cancer effect from D5D knockdown, DGLA supplementation along with 5-FU in 4 T1 cells. a Western blot of procaspase 9, PARP, cleaved PARP, acetyl histone H3, and γH2AX from D5D-KD 4 T1 cells treated with 5-FU (50 μM), DGLA (100 μM) and 5-FU + DGLA at 48 h. Protein expression rate was normalized using β-actin as a loading control; and b Western blot of MMP-2, MMP-9, E-cadherin, vimentin and snail from D5D-KD 4 T1 cells treated with 5-FU (50 μM), DGLA (100 μM) and 5-FU + DGLA at 48 h. Protein expression rate was normalized using β-actin as a loading control. Data represent as mean ± standard deviation (*: significant difference vs. control with p < 0.05; and #: significant difference vs. 5-FU group with p < 0.05 from n ≥ 3)

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