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Table 2 Characterization of organoids and cell lines

From: Development of primary human pancreatic cancer organoids, matched stromal and immune cells and 3D tumor microenvironment models

Tissue Bank #

Source

2D Culture

Days to Split

KRAS mutation

PDX1

CK19

Maspin

CRR9

αSMA

Vimentin

2112

primary

N

7

  

+

 

+

  

1914

liver met

N

7

  

+

 

+

  

2220

primary

N

3

  

+

 

+/−

  

2423

lung met

Y

5

G12 V

+

+(+)

+(2.7)

+(3)

-(−)

–

1710

ascites

Y

1

G12D

–

+

+

+

–

–

RA012

rapid autopsy

Y

27

WT

+

+

-(−)

+

–

–

RA012 met

rap. Aut. met

N

20

WT

+

+

 

+

–

–

2534

omentum

N

3

G12D

+

+(+)

+(2.7)

+(3)

-(−)

–

2475

liver met

N

17

WT

–

+(+)

+(1.0)

+(3)

-(−)

–

2535

peritoneum

N

5

G12 V

+

+(+)

(2.7)

+(3)

-(−)

–

2423CAF

CAFs

Y

 

WT

 

–

 

–

+*(+)

+

RA012CAF

CAFs

Y

 

WT

 

–

 

–

+*

+

  1. normal adj. = tumor adjacent normal tissue. Rap. aut. Met = metastatic tumor from rapid autopsy. Blank – not determined. *αSMA was positive is a subpopulation in co-culture with organoids. Tumor tissue staining indices are in parentheses (IHC: 1 = weak, 2 = moderate, 3 = strong, multiplied by percentage of positive cells)