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Table 2 Characterization of organoids and cell lines

From: Development of primary human pancreatic cancer organoids, matched stromal and immune cells and 3D tumor microenvironment models

Tissue Bank # Source 2D Culture Days to Split KRAS mutation PDX1 CK19 Maspin CRR9 αSMA Vimentin
2112 primary N 7    +   +   
1914 liver met N 7    +   +   
2220 primary N 3    +   +/−   
2423 lung met Y 5 G12 V + +(+) +(2.7) +(3) -(−)
1710 ascites Y 1 G12D + + +
RA012 rapid autopsy Y 27 WT + + -(−) +
RA012 met rap. Aut. met N 20 WT + +   +
2534 omentum N 3 G12D + +(+) +(2.7) +(3) -(−)
2475 liver met N 17 WT +(+) +(1.0) +(3) -(−)
2535 peritoneum N 5 G12 V + +(+) (2.7) +(3) -(−)
2423CAF CAFs Y   WT    +*(+) +
RA012CAF CAFs Y   WT    +* +
  1. normal adj. = tumor adjacent normal tissue. Rap. aut. Met = metastatic tumor from rapid autopsy. Blank – not determined. *αSMA was positive is a subpopulation in co-culture with organoids. Tumor tissue staining indices are in parentheses (IHC: 1 = weak, 2 = moderate, 3 = strong, multiplied by percentage of positive cells)