|
Tissue Bank #
|
Source
|
2D Culture
|
Days to Split
|
KRAS mutation
|
PDX1
|
CK19
|
Maspin
|
CRR9
|
αSMA
|
Vimentin
|
|---|
|
2112
|
primary
|
N
|
7
| | |
+
| |
+
| | |
|
1914
|
liver met
|
N
|
7
| | |
+
| |
+
| | |
|
2220
|
primary
|
N
|
3
| | |
+
| |
+/−
| | |
|
2423
|
lung met
|
Y
|
5
|
G12 V
|
+
|
+(+)
|
+(2.7)
|
+(3)
|
-(−)
|
–
|
|
1710
|
ascites
|
Y
|
1
|
G12D
|
–
|
+
|
+
|
+
|
–
|
–
|
|
RA012
|
rapid autopsy
|
Y
|
27
|
WT
|
+
|
+
|
-(−)
|
+
|
–
|
–
|
|
RA012 met
|
rap. Aut. met
|
N
|
20
|
WT
|
+
|
+
| |
+
|
–
|
–
|
|
2534
|
omentum
|
N
|
3
|
G12D
|
+
|
+(+)
|
+(2.7)
|
+(3)
|
-(−)
|
–
|
|
2475
|
liver met
|
N
|
17
|
WT
|
–
|
+(+)
|
+(1.0)
|
+(3)
|
-(−)
|
–
|
|
2535
|
peritoneum
|
N
|
5
|
G12 V
|
+
|
+(+)
|
(2.7)
|
+(3)
|
-(−)
|
–
|
|
2423CAF
|
CAFs
|
Y
| |
WT
| |
–
| |
–
|
+*(+)
|
+
|
|
RA012CAF
|
CAFs
|
Y
| |
WT
| |
–
| |
–
|
+*
|
+
|
- normal adj. = tumor adjacent normal tissue. Rap. aut. Met = metastatic tumor from rapid autopsy. Blank – not determined. *αSMA was positive is a subpopulation in co-culture with organoids. Tumor tissue staining indices are in parentheses (IHC: 1 = weak, 2 = moderate, 3 = strong, multiplied by percentage of positive cells)
