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Fig. 4 | BMC Cancer

Fig. 4

From: MiR-30a-5p confers cisplatin resistance by regulating IGF1R expression in melanoma cells

Fig. 4

IGF1R can regulate AKT activity and protein level of P53. a IGF1R siRNAs were transfected into M8 and SK-Mel-19 cells for 24 h, then the mRNA expression level of IGF1R was detected by real-time PCR. b After 12 h of silencing IGF1R, cells were incubated with the indicated concentrations of cisplatin for 72 h and subsequently subjected to MTS assay. c The protein level of AKT, P-AKT, P53 in M8, M8/DDP, SK-Mel-19 and SK-Mel-19/DDP cells. d IGF1R siRNAs were transfected into M8 and SK-Mel-19 cells for 48 h, then the protein levels of IGF1R, AKT, P-AKT, P53 were detected by Western blotting analysis

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