Fig. 3
From: MiR-30a-5p confers cisplatin resistance by regulating IGF1R expression in melanoma cells
IGF1R is a direct target of miR-30a-5p. a The 3’-UTR of IGF1R contains two predicted miR-30a-5p binding sites. The alignment of the seed regions of miR-30a-5p with IGF1R 3’-UTRs is shown in the figure. The sites of target mutagenesis are indicated in red underline. b The mRNA expression level of IGF1R in M8, M8/DDP, SK-Mel-19 and SK-Mel-19/DDP cells was detected by real-time PCR analysis. c The protein expression level of IGF1R in M8, M8/DDP, SK-Mel-19 and SK-Mel-19/DDP cells was detected by Western blotting analysis. d After 24 h of transfection miR-30a-5p mimic or inhibitor in M8, M8/DDP, SK-Mel-19 and SK-Mel-19/DDP cells, the expression of IGF1R was detected by real-time PCR. e After 48 h of transfection miR-30a-5p mimic or inhibitor in M8, M8/DDP, SK-Mel-19 and SK-Mel-19/DDP cells, the expression of IGF1R was detected by Western blotting analysis. f HEK293T cells were co-transfected with psiCHECK-2 reporter containing a wild type or mutated miR-30a-5p binding site shown in Fig. 3a together with control or miR-30a-5p mimic. Luciferase activities were measured after 24 h of transfection