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Fig. 2 | BMC Cancer

Fig. 2

From: MiR-30a-5p confers cisplatin resistance by regulating IGF1R expression in melanoma cells

Fig. 2

MiR-30a-5p affects the resistance of melanoma cells to cisplatin. a After 12 h of transfection miR-30a-5p mimic or inhibitor, cells were incubated with the indicated concentrations of cisplatin for 72 h and subsequently subjected to MTS assay. b Construction of the Plent-U6-GFP-Puro vector expressed TuD-miR-30a-5p. c The knockdown efficiency of miR-30a-5p in M8/DDP and SK-Mel-19/DDP cells was detected by real-time PCR. d MiR-30a-5p knockdown and vector control cells were treated with different concentrations of cisplatin for 72 h. MTS assay was used to detect cell proliferation

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