Fig. 4

Autophagic inhibition promoted delphinidin-induced apoptosis in MDA-MB-453 cells. a Expression of GFP-LC3-positive punctuate dots under autophagy inhibition by 3-MA (× 400). Statistical graph of GFP-LC3-positive dots. b Expression of GFP-LC3-positive punctuate dots under autophagy inhibition by BA1 (× 400). Statistical graph of GFP-LC3-positive dots. c The expression of LC3-II protein under autophagy inhibition by 3-MA. Statistical analysis of the LC3-II/LC3-I ratio. d The expression of LC3-II protein under autophagy inhibition by BA1. Statistical analysis of the LC3-II/LC3-I ratio. e CCK-8 assay to evaluate MDA-MB-453 cells viability under autophagy inhibition by 3-MA and BA1 respectively. f Expression of TUNEL-positive MDA-MB-453 cells after autophagic inhibition by 3-MA. g Expression of TUNEL-positive MDA-MB-453 cells after autophagic inhibition by BA1. h Statistical graph of TUNEL-positive MDA-MB-453 cells under autophagy inhibition by 3-MA and BA1. The concentration of Dp was 80 μM. Control: DMSO (0.1%). 3-MA: 5 mM. BA1: 160 nM. Dp: delphinidin. Data were obtained from three independent experiments. *P < 0.05 vs control group