Fig. 6From: Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cellsChanges in the expression of the CLK family of splice factor kinases in response to hypoxia and the effect of CLK1 inhibition on caspase 9 splicing. a PC3 cells were exposed for 16Â h to three different hypoxic conditions; 1.0%, 0.2% and 0.0% oxygen and N (normoxia). The relative expression of CLK1 and of its homologue CLK3 are shown, normalised to the housekeeping gene RPL31A. b PC3 cells were treated for 48Â h with the CLK1 inhibitor TG003; the effect on alternative splicing of CASP9 is shown. c Microarray time-course showing the expression of four CLKs over 24Â h in 0% hypoxia in DU145 (prostate), HT29 (colon) and MCF7 (breast) cancer cell linesBack to article page