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Fig. 4 | BMC Cancer

Fig. 4

From: Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cells

Fig. 4

Verification of high-throughput PCR results. RNA was extracted from PC3 (N = 5) and VCaP (N = 3) prostate cancer, and PNT2 (N = 3) normal prostate epithelium cell lines after 48 h in normoxia or 1% in hypoxia. For each gene a representative PCR is shown, together with the cassette exon involved. The average splicing index ψ (proportion of exon included) is shown graphically. The genes are RAP1GDS1, PTPN13, TTC23 and CASP9

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