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Fig. 1 | BMC Cancer

Fig. 1

From: Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cells

Fig. 1

Exposure of PC3 cells to hypoxia (1% oxygen) results in changes in alternative splicing of cancer associated genes. PC3 prostate cancer cells were exposed to 1% oxygen or normoxic conditions for up to 48 h. a Protein samples were extracted at 24 and 48 h time points and western blotted for CA IX (carbonic anhydrase 9), a hypoxia marker; N refers to normoxia and H to hypoxia. β-actin was used as a loading control. b RNA samples were extracted at 48 h and sent to the RNomics platform at the University of Sherbrooke, Canada, for high-throughput PCR analysis of the rate of inclusion of 600 cancer-associated cassette exons. Genes whose ψ indexes (proportion of exon included) changed by > 25% are listed

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