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Fig. 3 | BMC Cancer

Fig. 3

From: The DNA methylation profile of liver tumors in C3H mice and identification of differentially methylated regions involved in the regulation of tumorigenic genes

Fig. 3

Slpi associated with hypo-DMR is up-regulated in the liver tumor tissues of C3H mice. a Schematic view of the Slpi locus. The position of TSS, DMR and the position assessed by bisulfite sequencing (BS) were indicated. The magnified view indicates 7 CpG positions in the DMR and predicted transcription factor binding sites. b The average methylation values of the CpG sites in normal and tumor tissues. The CpG sites with ≥10 reads on both strands are indicated. Statistical significance between normal and tumor tissue was analyzed by Student’s t-test. * Significantly different at p < 0.05. cValidation of RRBS data for normal and tumor liver tissues of C3H mice by bisulfite sequencing. Methylated and unmethylated cytosine are shown as and respectively. d Validation of gene expression levels of Slpi by real-time PCR in the normal (n = 6) and tumor tissues (n = 11) of C3H mice. The expression of Slpi is normalized to the expression of rRNA. Statistical significance between the two groups was analyzed by the Student’s t-test. *** Significantly different at p < 0.001

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