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Fig. 2 | BMC Cancer

Fig. 2

From: The DNA methylation profile of liver tumors in C3H mice and identification of differentially methylated regions involved in the regulation of tumorigenic genes

Fig. 2

Mst1r associated with hypo-DMR is up-regulated in the liver tumor tissues of C3H mice. a Schematic view of the Mst1r locus. Methylation levels of CpG sites were visualized using Integrative Genomics Viewer (IGV) software. The position of TSS, DMR and the positions assessed by bisulfite sequencing (BS-1 and BS-2) were indicated. The magnified view indicates 8 CpG positions in the DMR and predicted transcription factor binding sites. b The average methylation values of the CpG sites in normal and tumor tissues. The CpG sites with ≥10 reads on both strands (≥20 reads in total) are indicated. Statistical significance between normal and tumor tissue was analyzed by Student’s t-test. **, *** Significantly different at p < 0.01 and 0.001, respectively. c Validation of RRBS data for normal and tumor liver tissues of C3H mice by bisulfite sequencing. Methylated and unmethylated cytosine are shown as and respectively. The numbers above the circles indicate the position of CpG shown in Fig. 1a. d Validation of gene expression levels of Mst1r by real-time PCR in the normal (n = 6) and tumor tissues (n = 11) of C3H mice. The expression of Mst1r is normalized to the expression of rRNA. Statistical significance between the two groups was analyzed by Student’s t-test. *** Significantly different at p < 0.001

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