Fig. 6

Double-immunofluorescence stainings of glioblastoma biopsies and spheroids. a-c Co-expression of TIMP-1 and CD63, d-f co-expression of TIMP-1 and the tumor stem cell-related marker CD133, and g-i co-expression of TIMP-1 and the tumor stem cell-related marker Sox2 in glioblastoma biopsies (a, d, g), cultured organotypic primary spheroids (b, e, h) and U87MG cell line-derived spheroids (c, f, i). TIMP-1/CD63 co-expression was detected in the biopsies (a) and spheroids (b, c). Only some tumor cells co-expressed TIMP-1 and CD133 in the biopsies (d) and spheroids (e, f). TIMP-1/Sox2 co-expression was detected in some tumor cells in the biopsies (g) and in the primary spheroids (h), whereas no Sox2 expression was detected in the U87MG cell line derived spheroids (i). Scale bar: 30 μm