Fig. 2
From: Sepsis increases perioperative metastases in a murine model
Hypothermia does not increase metastatic disease. a Experimental overview. BALB/c mice were injected intravenously (IV) through the tail vein with 3 × 105 CT26lacZ cells. Mice undergoing hypothermia were placed directly on the metal block (shown) while control mice were kept under normothermic conditions with heating pads. One hour into anesthesia, surgical stress (Sx) was generated by laparatomy (Lap). Mice were removed from anesthesia after 2 h total and kept in normal housing conditions. Temperature was measured during anesthesia using a rectal probe (as shown). Mice were sacrificed at 72 h to quantify lung metastases. b Maintenance of body temperature. Mice kept under normothermic conditions alone ranged from 31.88 °C to 38.16 °C in comparison to hypothermic conditions which ranged from 23.98 °C to 32.75 °C for 100 min. c Hypothermia alone does not increase lung metastases. The impact of body temperature upon lung metastases was assessed in mice maintained at normothermic (n = 8) and hypothermic (n = 8) conditions. d Prometastic effects of surgical stress are not impacted by hypothermic conditions. Lung metastases were measured in mice that did not undergo surgery (No Sx, n = 5) and mice undergoing a laparotomy under normothermic (Lap, n = 4) and hypothermic conditions (Hypothermic + Lap, n = 5). Error bars represent ± SEM