Fig. 4

Real-time PCR analysis for cell cycle-related genes. The OUMS-27 cells were treated with 10 μM CGP for 24 h. Total RNA was extracted and cDNA was synthesized by reverse transcription. Primers for genes involved in cell cycle regulation were used and real-time PCR was performed. Data were corrected against GAPDH values. ** indicates significant differences between the control and each group (P < 0.01). *** indicates significant differences between the control and each group (P < 0.001). Data are presented as the mean ± SD