Fig. 3

Western blot analysis for AKT/PI3K and MAPKs pathways. The levels of (a) GAPDH, ERK, and phospho-ERK in OUMS-27 cells treated with 100 μΜ BFN or 10 μΜ CGP (b) p38, phospho-p38, JNK, and phospho-JNK, and (c) total AKT, phospho-AKT (Th308) and phospho-AKT (Ser 473) treated with 10 μΜ CGP. The analysis was performed using respective primary antibodies and HRP-linked secondary antibodies. Protein levels were detected using a lumino image analyzer