Fig. 2

Apoptosis and cell cycle of OUMS-27 cells in vitro. a Flow cytometric analysis of apoptosis. OUMS-27 cells were treated with the indicated concentrations of CGP. Apoptotic cells were analyzed by FACScan flow cytometry. * indicates significant differences between the control and each group (P < 0.05). ** indicates significant differences between the control and each group (P < 0.01). b. Determination of caspase activity. OUMS-27 cells were treated with 10 μM CGP or DMSO. After 24 h of drug treatment, fluorescent intensities indicating cell viability and luminescent intensities indicating caspase-3, caspase-8, and caspase-9 activities were measured. Each estimate of caspase activity was adjusted with the corresponding cell viability. * indicates significant differences between the control and each group (P < 0.05). c. Determination of the percentage of cells in each phase of the cell cycle. OUMS-27 cells were treated with the indicated concentration of CGP for 24 h. The cell cycle distribution was measured by flow cytometry. * indicates significant differences between the control and each group (P < 0.05). ** indicates significant differences between the control and each group (P < 0.01). Data are presented as the mean ± SD