Fig. 1

Functional GJIC is established during clustering of MCF7 cancer cells. a Clustering of MCF7 cells in the absence of anchorage. Representative transmitted light microscopy images of the clustering assay at the indicated time points. b, c In experimental conditions identical to (a), analysis of fluorescent dye transfer between calcein-loaded cells (blue) and negative cells (black). (b) Flow cytometry quantification of fluorescence intensity. Newly positive cells (fluorescence intensity higher than the threshold indicated by the dotted red vertical line) are identified as receiver cells (green). c Quantification of the flow cytometry analysis. At each time point of the experiment, the percentage of cells in each category is indicated. Results are the mean ± SD of 4 independent experiments with at least 3 replicates per conditions. Unpaired two-tailed t-tests, *** p < 0.0005