Fig. 2
From: The effects of trastuzumab on HER2-mediated cell signaling in CHO cells expressing human HER2
Binding of trastuzumab to HER receptors in CHO-K6, CHO-EGFR and CHO-HER3 cells as revealed by immunofluorescence. a and b The binding of trastuzumab to HER2 in CHO-K6 cells. CHO cells were treated with trastuzumab at various concentrations for 1 h (a) or at various time period at 10 μg/ml (b) as indicated. The membrane localization (binding) of trastuzumab was revealed by TRITC-conjugated donkey anti-human IgG. The localization of HER2 was revealed by rabbit anti-HER2 antibody followed by FITC-conjugated donkey anti-rabbit IgG. The cell nuclei were counter stained with DAPI. Yellow indicated the co-localization of trastuzumab and HER2. c The binding of trastuzumab to EGFR in CHO-EGFR cells. The membrane localization (binding) of trastuzumab was revealed by TRITC-conjugated donkey anti-human IgG. The localization of EGFR was revealed by rabbit anti-EGFR antibody followed by FITC-conjugated donkey anti-rabbit IgG. The cell nuclei were counter stained with DAPI. d The binding of trastuzumab to HER3 in CHO-HER3 cells. The membrane localization (binding) of trastuzumab was revealed by TRITC-conjugated donkey anti-human IgG. The localization of HER3 was revealed by rabbit anti-HER3 antibody followed by FITC-conjugated donkey anti-rabbit IgG. The cell nuclei were counter stained with DAPI. Size bar: 10 μm