Fig. 4
From: Inhibition of autophagy sensitizes cancer cells to Photofrin-based photodynamic therapy
Elimination of ATG5-dependent autophagy sensitizes cells to PDT. a HeLa-sgGFP and HeLa-sgATG cells were subjected to in vitro PDT and the cell survival was determined by crystal violet staining 24 h after treatment. The viability in each experimental group is calculated versus its own untreated control. Data represent the mean values of 2 independent experiments ± S.D. (represented by error bars), **p < 0.001 (Student’s t-test). b To confirm impaired autophagic flux, cells were treated as in (a) and the whole-cell lysates were collected and ATG5 and LC3 levels were analyzed by Western blotting. c Immunofluorescence microscopy was used to confirm abrogated autophagosome formation after PDT