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Fig. 2 | BMC Cancer

Fig. 2

From: Inhibition of autophagy sensitizes cancer cells to Photofrin-based photodynamic therapy

Fig. 2

ShRNA-mediated ATG5 downregulation moderately affects autophagy and PDT efficacy. a HeLa and MCF-7 cells were infected with lentiviral particles containing shRNA targeting ATG5 (shATG5) or scrambled shRNA (shNTC) and subsequently incubated with puromycin to isolate stable cell lines. The stable cell lines were subjected to in-vitro PDT and cell survival was determined 24 h post-PDT by crystal violet staining. The bars represent survival in each experimental versus its own untreated control. Data show the mean values of 2 independent experiments ± S.D. (represented by error bars), and *P < 0.05 (Student’s t-test) (b) Whole-cell lysates from shATG5 and shNTC cell lines were collected 24-h post PDT and ATG5 and LC3 expression was evaluated by Western blotting. β-actin expression was assessed as protein loading control

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