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Fig. 1 | BMC Cancer

Fig. 1

From: Inhibition of autophagy sensitizes cancer cells to Photofrin-based photodynamic therapy

Fig. 1

Photofrin-PDT induces autophagy. a HeLa (left panel) and MCF-7 (right panel) cells were incubated with Photofrin for 24 h before exposure to different light fluencies. Whole-cell lysates were collected at indicated time points after PDT and analyzed by Western blotting for LC3 and β-actin (loading control) expression. b The experiment was performed as in (a), but, for indicated samples, 10 μM chloroquine (CQ) was added to the culture medium after PDT. c 16 h after irradiations, the cells were fixed and stained with anti-LC3 antibody to visualize autophagosomes by immunofluorescence

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