Fig. 1
From: Inhibition of autophagy sensitizes cancer cells to Photofrin-based photodynamic therapy
Photofrin-PDT induces autophagy. a HeLa (left panel) and MCF-7 (right panel) cells were incubated with Photofrin for 24 h before exposure to different light fluencies. Whole-cell lysates were collected at indicated time points after PDT and analyzed by Western blotting for LC3 and β-actin (loading control) expression. b The experiment was performed as in (a), but, for indicated samples, 10 μM chloroquine (CQ) was added to the culture medium after PDT. c 16 h after irradiations, the cells were fixed and stained with anti-LC3 antibody to visualize autophagosomes by immunofluorescence