Fig. 1

Up-regulated of TRPC5 expression and increased glycolysis in 5-Fu chemoresistant human CRC cells. a MTT assay showed that HCT-8/5-Fu and LoVo/5-Fu cells were much more resistant to 5-Fu-induced cell death than HCT-8 and LoVo cells. Administration of 3-BP or 2DG caused a remarkable reversal of 5-Fu resistance in HCT-8/5-Fu and LoVo/5-Fu cells, while caused no obvious change to HCT-8 cells (n = 6, *p < 0.05, Student’s t-test). Real-time PCR (b) and western blot (c) showed much higher expression of both TRPC5 and GLUT1 at the mRNA and protein levels in HCT-8/5-Fu than in HCT-8 cells (n = 4, *p < 0.05, Student’s t-test). d Higher glucose consumption rates in HCT-8/5-Fu cells than in HCT-8 cells (n = 4, *p < 0.05, Student’s t-test). e More ATP production in HCT-8/5-Fu than in HCT-8 cells (n = 4, *p < 0.05, Student’s t-test)