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Fig. 1 | BMC Cancer

Fig. 1

From: Oxidative damage and response to Bacillus Calmette-Guérin in bladder cancer cells expressing sialyltransferase ST3GAL1

Fig. 1

BCG-stimulation of HT1376 cells. a: Secretion of IL-6 and IL-8. HT1376T, (white circles) and HT1376sT (black circles) cells were challenged with BCG for 2 h, then they were washed and cultured in medium for 16 h. Cytokine concentration was determined as described in Materials and Methods section. Data of three independent experiments are reported. Statistical analysis is reported in Additional file 2. b: Time course of BCG uptake. HT1376T (white circles) and HT1376sT (black circles) cells were challenged with CMTMR-labelled BCG for 2 and 6 h, as described in the Methods section. BCG internalization was estimated as mean fluorescence intensity of the cells, measured by flow cytometry Data of three independent experiments are reported

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