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Fig. 7 | BMC Cancer

Fig. 7

From: A novel miR-375-HOXB3-CDCA3/DNMT3B regulatory circuitry contributes to leukemogenesis in acute myeloid leukemia

Fig. 7

The anti-leukemia effects of miR-375 in vivo. About 1х107 viable HL-60 cells transduced with MSCV-miR-375 or MSCV-NC were injected subcutaneously into right flank of each nude mouse. a A photograph of xenografted tumors in mice xenografted by HL-60 cells, which were transduced with MSCV-miR-375 or MSCV-NC. b Volumes of all xenografted tumors were measured when the experiment was terminated at 42 days after tumor cell inoculation. c Net weights of all xenografted tumors were measured at the termination of the experiment. d The protein expression of HOXB3 was detected in xenografted tumor lysates from HL-60 cells transduced with MSCV-miR-375 or MSCV-NC. e THP1 cells were transduced with lentivirus vector pLVX-IRES-ZsGreen1 and GFP-positive cells were sorted by flow cytometry. The GFP-positive cells were further transduced with MSCV-miR-375 or MSCV-NC and treated with puromycin for 1 week. Then, THP1-GFP-miR-375 or THP1-GFP-NC were xenografted into NSG mice. Peripheral blood cells were extracted from mice when the mice became moribund and GFP-positive cells were analyzed by flow cytometry. *P < 0.01 versus MSCV-NC. Shown is a representative plot for GFP-positive cells (Left) and summary of GFP-positive cells (Right). f Representative images of spleens were observed from THP1-miR-375-engrafted mice or THP1-NC-engrafted mice (Left). All the spleens in THP1-miR-375-engrafted mice or THP1-NC-engrafted mice were weighted (Right). g MiR-375 prolonged the overall survival time in an engrafted mice model

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