Fig. 4

Ectopic expression of miR-375 inhibits cell growth and reduces colony formation. a Viable cell number was counted by the trypan-blue exclusion assay in HL-60 and THP1 cells, which were transduced with MSCV-miR-375 or MSCV-NC for the indicated times. *P < 0.01 versus MSCV-NC. b and c HL-60, THP1, and six primary AML blasts were transduced with MSCV-miR-375 or MSCV-NC, and then plated in methylcellulose for leukemia progenitor cell colony formation. *P < 0.01 and ^#P < 0.05 versus MSCV-NC. d Normal mouse bone marrow cells were transduced with MSCV-GFP-NC, MSCV-GFP-MLL-AF9, or MSCV-GFP-MLL-AF9+ MSCV-miR-375, and colony forming/replating assays were done thereafter. *P < 0.01. e Colony formation was counted in CD34⁺ hematopoietic stem and progenitor cells (HSPC), which were freshly isolated from three umbilical cord blood (UCB) and transduced with MSCV-NC or MSCV-miR-375. f HOXB3 protein level was measured in HL-60 and THP1 cells transduced with LVX-NC or LVX-HOXB3 for 48 h. g The rescue experiments were performed in HL-60 and THP1 cells, which were transduced with MSCV-NC, MSCV-miR-375, MSCV-miR-375 plus LVX-NC, or MSCV-miR-375 plus LVX-HOXB3, respectively. *P < 0.01 and ^#P < 0.05. h Colony formation was performed in HL-60 and THP1 cells following transduction with MSCV-NC, MSCV-miR-375, MSCV-miR-375 plus LVX-NC, or MSCV-miR-375 plus LVX-HOXB3, respectively. *P < 0.01