Table 1 Primers used for LAMP amplification of OPSCC specimens (5′ to 3′)
HPV 16a | F3 | TCGGTTGTGCGTACAAAG |
B3 | AGCCTCTACATAAAACCATCC | |
FIP | TGGGGCACACAATTCCTAGT*CACACACGTAGACATTCGT | |
BIP | TCAGAAACCATAATCTACCATGGC*ATTACATCCCGTACCCTCTT | |
LF | CCCATTAACAGGTCTTCCAAAGT | |
LB | CCTGCAGGTACCAATGGGG | |
HPV 18a | F3 | AACGACGATTCCACAACA |
B3 | CAACCGGAATTTCATTTTGG | |
FIP | GTCTTTCCTGTCGTGCTCGGT*AGCTGGGCACTATAGAGG | |
BIP | CGACGCAGAGAAACACAAGT*CTCTAAATGCAATACAATGTCTTG | |
LF | CAGCACGAATGGCACTGG | |
LB | TATTAAGTATGCATGGACCTAAGGC | |
HPV 31 | F3 | AGAAGAAAAACAAAGACATTTGG |
B3 | CTCCTCATCTGAGCTGTC | |
FIP | GTCTTCTCCAACATGCTATGCA*GAAACGATTCCACAACATAGG | |
BIP | GAGAAACACCTACGTTGCAAGA*GGGTAATTGCTCATAACAGTG | |
LF | ACGTCCTGTCCACCTTCCT | |
LB | TGTGTTAGATTTGCAACCTGAGGCA | |
HPV 35 | F3 | TGCATGGAGAAATAACTACATTG |
B3 | CGCCTCACATTTACAACAG | |
FIP | TGTCACACAATTGCTCATAACAGTA*CAAGACTATGTTTTAGATTTGGAAC | |
BIP | GCTCAGAGGAGGAGGAAGATAC*GACGTTACAATATTATAATTGGAGG | |
LF | TATTGACGGTCCAGCTGGACAA | |
LB | GGACAAGCAAAACCAGACA |