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Fig. 1 | BMC Cancer

Fig. 1

From: Assessing the performance of a Loop Mediated Isothermal Amplification (LAMP) assay for the detection and subtyping of high-risk suptypes of Human Papilloma Virus (HPV) for Oropharyngeal Squamous Cell Carcinoma (OPSCC) without DNA purification

Fig. 1

Specificity of the LAMP detection of different HPV types: Purified plasmid DNA containing genomic inserts extracted from HPV 16, HPV 18, HPV 31, HPV 35a, and HPV 35b were amplified using HPV 16 (a), HPV 18 (b), HPV 31 (c), and HPV 35 (d) type-specific LAMP primers. The detection of LAMP products was done by 2% agarose gel electrophoresis, visual assessment of precipitate formation in the reaction tube, and the presence (+) or absence (−) of spectrophotometer absorbance at 400 nm compared to negative controls. Lane M: 100 bp marker, lane N: negative control, and number: HPV types

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BMC Cancer

ISSN: 1471-2407

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