Fig. 5

Plocabulin inhibits spheroid sprouting. a Representative images (upper panel) and quantification (lower panel) of spheroid sprouting assay as measured as cumulative sprout length (μm), sprout length (μm) and sprout number in the indicated conditions; data are shown as mean +/− standard deviation. b Representative images (upper panel) and quantification (lower panel) of spheroid sprouting assay in the presence of thymidine (T) as measured as cumulative sprout length (μm), sprout length (μm) and sprout number in the indicated conditions; data are shown as mean +/− standard deviation. Comparisons between different samples were analyzed by Student’s t test. Differences were considered significant at *P < 0.05, **P < 0.01 and ***P < 0.001 . Scale bars represent 100 μm