Fig. 4

Effects of ATO on AGS cells were reversed by tranfection with SHP-1 siRNA. a. Wound closure assay. Left panel; representative images of wound closure. Right panel; analysis of vertical wound distance. Data are presented as mean ± standard deviation. All experiments were performed in triplicate. ^*P < 0.05, compared with control; ^#P < 0.05, compared with siControl + ATO 10 μM (n = 3). b. Matrigel invasion assay. Left panel; representative images of Matrigel invasion assay. Right panel; analysis of invading cells. The number of positive invading cells was counted under × 20 magnification. Data are presented as mean ± standard deviation. Cell counting was performed in at least 3 randomly selected separate areas. ^*P < 0.05, compared with control; ^#P < 0.05, compared with siControl + ATO 10 μM (n = 3). c. Western blotting. Whole cell lysate protein was extracted after transfection and ATO treatment. β-actin was used as an internal loading control