Table 2 Antibodies and their specifications
From: Diethylnitrosamine induces lung adenocarcinoma in FVB/N mouse
Primary antibody | Manufacturer | Catalog number | Source | Dilution |
|---|---|---|---|---|
Akt | Cell Signaling Technology, Danvers, MA | #4691 | rabbit | 1:1000 |
p-Akt (Thr308) | Cell Signaling Technology | #2965 | rabbit | 1:1000 |
p-Akt (Ser473) | Cell Signaling Technology | #4058 | rabbit | 1:1000 |
p-Erk 1/2 | Cell Signaling Technology | #4370 | rabbit | 1:1000 |
p-GSK3 α/β | Cell Signaling Technology | #9331 | rabbit | 1:500 |
p-S6 | Cell Signaling Technology | #2211 | rabbit | 1:1000 |
CDK4 | Neomarkers, Fremont, California, US | #MS-616 | mouse | 1:250 |
PCNA | Atlas antibodies, Stockholm, Sweden | HPA030522 | rabbit | 1:1000 |
p-Rb S780 | Cell Signaling Technology | #9307 | rabbit | 1:250 |
β-actin | Sigma-Aldrich, St. Louis, MO | A2228 | mouse | 1:5000 |