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Fig. 5 | BMC Cancer

Fig. 5

From: Contrasting roles of H3K4me3 and H3K9me3 in regulation of apoptosis and gemcitabine resistance in human pancreatic cancer cells

Fig. 5

Dysregulation of apoptosis regulatory mediators in human PDAC. a MiaPaCa2 and PANC1 cells were treated with verticillin A at the indicated concentrations for 2 days. Acid extraction was prepared from the cells and analyzed by Western blotting with the antibodies specific for the indicated methylated histones. The protein band intensities were quantified using NIH image J and shown at the right panel. The H3K9me3 protein level was normalized as the ratio over the intensity of Η3. b RNA was extracted from human pancreatic tumor tissues and adjacent normal pancreas tissues. The mRNA levels of the indicated genes were analyzed by qPCR using gene-specific primer with β-actin as internal control. c Total protein was prepared from human pancreatic tumor tissues (patients T1 and T2) and adjacent normal pancreas tissues (N1: adjacent normal tissue from patient T1; N2: adjacent normal tissues from patient T2), and then analyzed by Western blotting analysis using antibodies for the indicated proteins. The protein band intensities were quantified using NIH image J and normalized as the ratio over the intensity of β-actin. Column: mean; Bar: SD

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